Use of a newly developed rapid microbial ATP bioluminescence assay to detect microbial contamination on poultry carcasses.

An Adenosine Triphosphate Bioluminescence Method for Evaluating the Microbial Contamination of the Salad-Preparing Tables and Salad-Serving Dishes in Restaurants of Mashhad City, Iran

Introduction: Consumption of vegetable products is increasing commonly in the world because they are recognized as an important source of nutrients, vitamins, and fiber for humans. Salads are among the most widely used foods that are also known as the most contaminated foods in restaurants. This study was conducted to determine the microbial contamination of salad-preparing tables and salad-ser...

Scientific Committee on Health and Environmental Risks SCHER Scientific Committee on Emerging and Newly Identified Health Risks SCENIHR Environmental impact and effect on antimicrobial resistance of four substances used for the removal of microbial surface contamination of poultry carcasses

In Situ Rapid Evaluation of Indoor Bioaerosols Using an ATP Bioluminescence Assay

An adenosine-5’-triphosphate (ATP) bioluminescence method was developed for detecting microbial activity in indoor air. This method was compared with the traditional method of collecting, culturing and counting CFUs. A comparison of the results showed that ATP bioluminescence, expressed as RLUs, was moderately correlated with the entire set of CFU counts (r = 0.607), and that the correlation im...

Identification of Salmonella spp. and Salmonella typhimurium by a multiplex PCR-based assay from poultry carcasses in Mashhad- Iran

Poultry meat has been identified as one of the principal foodborne sources of Salmonella. In this preliminary study the prevalence of Salmonella spp. and its typhimurium serovar contamination of broiler carcasses, were determined. Using the rinse test method, numbers of 60 samples, representing 20 broiler flocks, were collected from poultry carcasses after the chilling stage in the processing l...

Rapid identification of Campylobacter jejuni from poultry carcasses and slaughtering environment samples by real-time PCR.

The objective of this study was to develop a real-time PCR assay for rapid identification of Campylobacter jejuni and to apply the method in analyzing samples from poultry processing. A C. jejuni-specific primer set targeting a portion of the C. jejuni hippuricase gene was developed. The specificity of the newly designed primer pair was verified using 5 C. jejuni strains and 20 other bacterial ...